![]() ![]() This tutorial assumes that you have carried your gel or blot through the visualization step, so that you have a digital image of your gel in. ImageJ ( ) can be used to compare the density (aka intensity) of bands on an agar gel or western blot. The Image Studio Lite software can be downloaded for free from In addition to the functionality described below for ImageJ, Image Studio Lite provides additional data management features along with annotation tools for producing publication-ready images of your westerns. If you’re looking for a more comprehensive workflow option for your western blot analyses, please visit my tutorial on using Image Studio Lite, a free software package from LI-COR Biosciences. ![]() The following information is an updated version of a method for using ImageJ to analyze western blots from a now-deprecated older page. ![]()
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